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linear polyethyleneimine 25 kda  (Kyfora Bio)


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    Structured Review

    Kyfora Bio linear polyethyleneimine 25 kda
    Linear Polyethyleneimine 25 Kda, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 99/100, based on 2205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyethyleneimine/PEI+25K+Transfection+Reagent/pmc13049938-268-11-15
    Average 99 stars, based on 2205 article reviews
    linear polyethyleneimine 25 kda - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Expressing:

    Article Title: Structure of zebrafish NLRP3 reveals a novel mode of inflammasome activation
    Article Snippet: .. For protein expression, one liter of Expi293F cells with a density of 3×10 6 cells/ml were transfected with 1 mg plasmid using 1 mg polyethyleneimine (Polysciences, 23966-1) as transfection reagent. ..

    Transfection:

    Article Title: Structure of zebrafish NLRP3 reveals a novel mode of inflammasome activation
    Article Snippet: .. For protein expression, one liter of Expi293F cells with a density of 3×10 6 cells/ml were transfected with 1 mg plasmid using 1 mg polyethyleneimine (Polysciences, 23966-1) as transfection reagent. ..

    Article Title: Structure of zebrafish NLRP3 reveals a novel mode of inflammasome activation
    Article Snippet: .. After 24 h cells were transfected with plasmids encoding mScarlet-tagged NLRP3 constructs using polyethyleneimine (Polysciences, 23966-1) as transfection reagent. ..

    Article Title: Self-organized hemanoids derived from human iPSCs create a niche that produces definitive extraembryonic hematopoiesis
    Article Snippet: .. At about 70–80% confluency, cells were transfected using 5 μg/mL polyethyleneimine (#23966, Polysciences). .. For transfection of a total of 10 plates, 60 μg AAV donor plasmid ( pAAV6_AAVS1_CD43_GFP ) and 220 μg helper plasmid pDGM6 (was a gift from David Russell, #110660, Addgene) were mixed with PEI in Opti-MEM (#3798570, Gibco).

    Article Title: Structure of zebrafish NLRP3 reveals a novel mode of inflammasome activation
    Article Snippet: .. FLAG-tagged NLRP3 and HA-tagged NEK7 constructs were co-expressed in 300 ml Expi293F cells transfected at the density 3×10 6 cells/ml with 140 μg of each plasmid and 560 μg polyethyleneimine (Polysciences, 23966-1) as transfection reagent. .. 72 h post-transfection cells were harvested by centrifugation, lysed in buffer containing 50 mM Tris, 150 mM NaCl, 5 mM MgCl 2 , 10% Glycerol, pH 7.5, supplemented with protease inhibitor (Sigma-Aldrich, 11836170001) and 2 mM β-mercaptoethanol by sonication (2 s on, 8 s off, 2 min total on, 40% power, Fisherbrand, 505).

    Article Title: Cardiac-Detargeted MyoAAV Enables Systemic Nrl-Mediated Fast Myofiber Remodeling and Hypertrophy Across Multiple Skeletal Muscles
    Article Snippet: To compare preparation methods with or without ultracentrifugation, viral particles were purified using the AAVpro Purification Kit Maxi (All Serotypes; Takara Bio) according to the manufacturer’s instructions. .. For the Nrl-based experiments, AAVs were generated by triple plasmid transfection of 293T cells using polyethyleneimine (PEI MAX; Polysciences). ..

    Article Title: The ISG Atlas: a loss-of-function analysis characterizes antiviral properties of interferon stimulated genes.
    Article Snippet: .. Polyethyleneimine (Polysciences, 24765) was used for transfection at a 3:1 ratio relative to the total DNA. ..

    Article Title: PIEZO1 is Required for Acute Myeloid Leukemia Progression and Leukemia Stem Cell Maintenance via HIF1A-SLC7A11 Axis-Mediated Ferroptosis Defense.
    Article Snippet: .. To generate lentiviral particles, HEK293T cells were subjected to co-transfection with the packaging vectors pSPAX2 and pMD2.G, employing polyethyleneimine (PEI, Polysciences, Cat24765-1) as the transfection reagent. ..

    Plasmid Preparation:

    Article Title: Structure of zebrafish NLRP3 reveals a novel mode of inflammasome activation
    Article Snippet: .. For protein expression, one liter of Expi293F cells with a density of 3×10 6 cells/ml were transfected with 1 mg plasmid using 1 mg polyethyleneimine (Polysciences, 23966-1) as transfection reagent. ..

    Article Title: Structure of zebrafish NLRP3 reveals a novel mode of inflammasome activation
    Article Snippet: .. FLAG-tagged NLRP3 and HA-tagged NEK7 constructs were co-expressed in 300 ml Expi293F cells transfected at the density 3×10 6 cells/ml with 140 μg of each plasmid and 560 μg polyethyleneimine (Polysciences, 23966-1) as transfection reagent. .. 72 h post-transfection cells were harvested by centrifugation, lysed in buffer containing 50 mM Tris, 150 mM NaCl, 5 mM MgCl 2 , 10% Glycerol, pH 7.5, supplemented with protease inhibitor (Sigma-Aldrich, 11836170001) and 2 mM β-mercaptoethanol by sonication (2 s on, 8 s off, 2 min total on, 40% power, Fisherbrand, 505).

    Article Title: Cardiac-Detargeted MyoAAV Enables Systemic Nrl-Mediated Fast Myofiber Remodeling and Hypertrophy Across Multiple Skeletal Muscles
    Article Snippet: To compare preparation methods with or without ultracentrifugation, viral particles were purified using the AAVpro Purification Kit Maxi (All Serotypes; Takara Bio) according to the manufacturer’s instructions. .. For the Nrl-based experiments, AAVs were generated by triple plasmid transfection of 293T cells using polyethyleneimine (PEI MAX; Polysciences). ..

    Construct:

    Article Title: Structure of zebrafish NLRP3 reveals a novel mode of inflammasome activation
    Article Snippet: .. After 24 h cells were transfected with plasmids encoding mScarlet-tagged NLRP3 constructs using polyethyleneimine (Polysciences, 23966-1) as transfection reagent. ..

    Article Title: Structure of zebrafish NLRP3 reveals a novel mode of inflammasome activation
    Article Snippet: .. FLAG-tagged NLRP3 and HA-tagged NEK7 constructs were co-expressed in 300 ml Expi293F cells transfected at the density 3×10 6 cells/ml with 140 μg of each plasmid and 560 μg polyethyleneimine (Polysciences, 23966-1) as transfection reagent. .. 72 h post-transfection cells were harvested by centrifugation, lysed in buffer containing 50 mM Tris, 150 mM NaCl, 5 mM MgCl 2 , 10% Glycerol, pH 7.5, supplemented with protease inhibitor (Sigma-Aldrich, 11836170001) and 2 mM β-mercaptoethanol by sonication (2 s on, 8 s off, 2 min total on, 40% power, Fisherbrand, 505).

    Concentration Assay:

    Article Title: A nanobody-based proteolysis-targeting chimera offers broad-spectrum protection against diverse influenza virus infections
    Article Snippet: Lipofectamine LTX Reagent (15338100) and Opti-MEM (CN 31985070) were obtained from Invitrogen. .. Polyethyleneimine (PEI; 24765, stock concentration 1 mg/mL) was purchased from Polysciences. .. SeaPlaque agarose (50100) was purchased from Lonza.

    Generated:

    Article Title: Cardiac-Detargeted MyoAAV Enables Systemic Nrl-Mediated Fast Myofiber Remodeling and Hypertrophy Across Multiple Skeletal Muscles
    Article Snippet: To compare preparation methods with or without ultracentrifugation, viral particles were purified using the AAVpro Purification Kit Maxi (All Serotypes; Takara Bio) according to the manufacturer’s instructions. .. For the Nrl-based experiments, AAVs were generated by triple plasmid transfection of 293T cells using polyethyleneimine (PEI MAX; Polysciences). ..



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